We present an integrated approach for the enriched detection of genes subject to cis-acting
variation in the mouse genome. Gene expression profiling was performed with lung tissue
from a panel of recombinant congenic strains (RCS) derived from A/J and C57BL/6J inbred
mouse strains. A multiple-regression model measuring the association between gene
expression level, donor strain of origin (DSO), and predominant strain background identified
over 1,500 genes (P< 0.05) whose expression profiles differed according to the DSO. This …

MATERIALS AND METHODS RCS panel. Tissues used in these studies were obtained from
a RCS panel derived from A/J and C57BL/6J parents (18). The mice used in this study were
part of a 600-animal experiment that took place over a 4-mo period. The mice used in the F1
validation study were reared in the same animal facility (with the same diet and housing
conditions). All animals were killed at 16 wk of age, and tissues were harvested according to
a standard dissection protocol. All mice were handled according to guidelines and …