Trypanosoma cruzi parasites are the causative agents of Chagas disease, a leading
infectious form of heart failure whose pathogenesis is still not fully characterized. In this
work, we applied untargeted liquid chromatography–tandem mass spectrometry to heart
sections from T. cruzi-infected and uninfected mice. We combined molecular networking and
three-dimensional modeling to generate chemical cartographical heart models. This
approach revealed for the first time preferential parasite localization to the base of the heart …