Measurement error and resolution in quantitative stable isotope probing: implications for experimental design
Quantitative stable isotope probing (qSIP) estimates isotope tracer incorporation into DNA of
individual microbes and can link microbial biodiversity and biogeochemistry in complex
communities. As with any quantitative estimation technique, qSIP involves measurement
error, and a fuller understanding of error, precision, and statistical power benefits qSIP
experimental design and data interpretation. We used several qSIP data sets—from soil and
seawater microbiomes—to evaluate how variance in isotope incorporation estimates …
individual microbes and can link microbial biodiversity and biogeochemistry in complex
communities. As with any quantitative estimation technique, qSIP involves measurement
error, and a fuller understanding of error, precision, and statistical power benefits qSIP
experimental design and data interpretation. We used several qSIP data sets—from soil and
seawater microbiomes—to evaluate how variance in isotope incorporation estimates …
[PDF][PDF] Measurement Error and Resolution in Quantitative Stable Isotope Probing: Implications
Quantitative stable isotope probing (qSIP) estimates isotope tracer incorporation into DNA of
individual microbes and can link microbial biodiversity and biogeochemistry in complex
communities. As with any quantitative estimation technique, qSIP involves measurement
error, and a fuller understanding of error, precision, and statistical power benefits qSIP
experimental design and data interpretation. We used several qSIP data sets—from soil and
seawater microbiomes—to evaluate how variance in isotope incorporation estimates …
individual microbes and can link microbial biodiversity and biogeochemistry in complex
communities. As with any quantitative estimation technique, qSIP involves measurement
error, and a fuller understanding of error, precision, and statistical power benefits qSIP
experimental design and data interpretation. We used several qSIP data sets—from soil and
seawater microbiomes—to evaluate how variance in isotope incorporation estimates …
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