Mechanical compressive force inhibits adipogenesis of adipose stem cells
Cell proliferation, 2013•Wiley Online Library
Objective The aim of this study was to investigate effects of mechanical compressive force
on differentiation of adipose‐derived stem cells (ASC s) in vitro. Materials and methods Mice
ASC s were treated with compressive force (2000 με, 1 Hz) for 2 or 6 h after adipogenic
induction for 3 days, then oil red O staining was used to examine oil droplet‐filled cells.
Adipogenic genes, PPAR‐γ1 and APN, were examined by real‐time PCR and
immunofluorescence (IF) staining was performed to test expression of de‐PPAR‐γ and ph …
on differentiation of adipose‐derived stem cells (ASC s) in vitro. Materials and methods Mice
ASC s were treated with compressive force (2000 με, 1 Hz) for 2 or 6 h after adipogenic
induction for 3 days, then oil red O staining was used to examine oil droplet‐filled cells.
Adipogenic genes, PPAR‐γ1 and APN, were examined by real‐time PCR and
immunofluorescence (IF) staining was performed to test expression of de‐PPAR‐γ and ph …
Objective
The aim of this study was to investigate effects of mechanical compressive force on differentiation of adipose‐derived stem cells (ASCs) in vitro.
Materials and methods
Mice ASCs were treated with compressive force (2000 με, 1 Hz) for 2 or 6 h after adipogenic induction for 3 days, then oil red O staining was used to examine oil droplet‐filled cells. Adipogenic genes, PPAR‐γ1 and APN, were examined by real‐time PCR and immunofluorescence (IF) staining was performed to test expression of de‐PPAR‐γ and ph‐PPAR‐γ at the protein level.
Results
Our data showed that mechanical compressive force reduced numbers of oil droplet‐filled cells, and down‐regulated mRNA levels of both PPAR‐γ1 and APN and protein level of PPAR‐γ, in ASCs.
Conclusions
In culture medium containing adipogenic stimuli, mechanical compressive force inhibited adipogenesis of ASCs.
Wiley Online Library以上显示的是最相近的搜索结果。 查看全部搜索结果