[HTML][HTML] Methods for RNA extraction, cDNA preparation and analysis of CFTR transcripts
AS Ramalho, S Beck, CM Farinha, LA Clarke… - Journal of Cystic …, 2004 - Elsevier
Journal of Cystic Fibrosis, 2004•Elsevier
The scope of this article is to outline some of the basic methods for good quality RNA
preparation from mammalian tissues and cells (including epithelial cells). Additionally, we
give an outline of common techniques of measuring CFTR gene expression such as
quantitative and semi-quantitative reverse transcription (RT) PCR and ribonuclease
protection assay (RPA). These methods are designed to detect low abundance transcripts,
which apply to CFTR mRNA in most cell types and tissues.
preparation from mammalian tissues and cells (including epithelial cells). Additionally, we
give an outline of common techniques of measuring CFTR gene expression such as
quantitative and semi-quantitative reverse transcription (RT) PCR and ribonuclease
protection assay (RPA). These methods are designed to detect low abundance transcripts,
which apply to CFTR mRNA in most cell types and tissues.
The scope of this article is to outline some of the basic methods for good quality RNA preparation from mammalian tissues and cells (including epithelial cells). Additionally, we give an outline of common techniques of measuring CFTR gene expression such as quantitative and semi-quantitative reverse transcription (RT) PCR and ribonuclease protection assay (RPA). These methods are designed to detect low abundance transcripts, which apply to CFTR mRNA in most cell types and tissues.
Elsevier
以上显示的是最相近的搜索结果。 查看全部搜索结果