Isolation, cultivation, and characterization of human mesenchymal stem cells

D Mushahary, A Spittler, C Kasper, V Weber… - Cytometry Part …, 2018 - Wiley Online Library
… As to the different sources and methods used for stem cell isolation, … stem cell derived EVs.
While literature data on these effects are still scarce, EVs secreted by MSC from different cell

Evaluation of methods for cultivating limbal mesenchymal stromal cells

LJ Bray, CF Heazlewood, K Atkinson, DW Hutmacher… - …, 2012 - Taylor & Francis
Mesenchymal stromal cells (MSC) with similar properties to bone marrow-derived mesenchymal
stromal cells (BM… We have evaluated methods for culturing human limbal MSC (L-MSC). …

Explant culture: An advantageous method for isolation of mesenchymal stem cells from human tissues

F Hendijani - Cell proliferation, 2017 - Wiley Online Library
Mesenchymal stem cell (MSC) research progressively moves towards clinical phases due …
method. A systematic search in PubMed using search key word “mesenchymal stem cell” and …

Collection and propagation methods for mesenchymal stromal cells

SE Taylor, PD Clegg - Veterinary Clinics: Equine Practice, 2011 - vetequine.theclinics.com
… , techniques to expand cell numbers in vitro have been developed. Several techniques for
… transferred to tissue culture flasks in a tissue culture hood and cultured with growth medium (…

Dynamic three-dimensional culture methods enhance mesenchymal stem cell properties and increase therapeutic potential

JE Frith, B Thomson, PG Genever - … Engineering Part C: Methods, 2010 - liebertpub.com
… (3D) techniques for in vitro MSC culture using spinner flasks … We characterized the two
methods for dynamic 3D MSC culturecellular spheroids and remain viable in dynamic 3D culture. …

A rapid and efficient method for expansion of human mesenchymal stem cells

SK Both, AJC Muijsenberg, CA Blitterswijk… - Tissue …, 2007 - liebertpub.com
… On the basis of these results we have developed an efficient method to culture hMSCs by
seeding the cells at 100 cells/cm2 in an a-minimal essential medium–based medium …

Evaluation of isolation methods and culture conditions for rat bone marrow mesenchymal stem cells

X Li, Y Zhang, G Qi - Cytotechnology, 2013 - Springer
… A are isolated using direct adherence method and cultured by conventional medium changing;
… of two methods of in vitro isolation of adult bone marrow mesenchymal stem cells. J Clin …

A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow

M Soleimani, S Nadri - Nature protocols, 2009 - nature.com
culture of mesenchymal stem cells (MSCs) from mouse bone marrow (BM) to supply researchers
with a method that can be applied in cell … change in primary culture and diminishing the …

[HTML][HTML] An improved protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow

S Huang, L Xu, Y Sun, T Wu, K Wang, G Li - Journal of orthopaedic …, 2015 - Elsevier
… of the cultured mouse bone marrow mesenchymal stem cells using our protocol. (A) On Day
1, most of the cells were … The cells established using our culture method are mesenchymal

Phenotypic and functional comparison of cultures of marrow‐derived mesenchymal stem cells (MSCs) and stromal cells

MK Majumdar, MA Thiede, JD Mosca… - Journal of cellular …, 1998 - Wiley Online Library
… isolation method and composition of the culture me- our analysis was not quantitative,
little can be said about any differences observed in the constitutive levels dium used in the …