We have previously demonstrated in H295R adrenocortical cells that the Ca²⁺-dependent production of mitochondrial cAMP (mt-cAMP) by the matrix soluble adenylyl cyclase (sAC; encoded by ADCY10) is associated with enhanced aldosterone production. Here, we examined whether mitochondrial sAC and mt-cAMP fine tune mitochondrial Ca²⁺ metabolism to support steroidogenesis. Reduction of mt-cAMP formation resulted in decelerated mitochondrial Ca²⁺ accumulation in intact cells during K⁺-induced Ca²⁺ signalling and also in permeabilized cells exposed to elevated perimitochondrial [Ca²⁺]. By contrast, treatment with the membrane-permeable cAMP analogue 8-Br-cAMP, inhibition of phosphodiesterase 2 and overexpression of sAC in the mitochondrial matrix all intensified Ca²⁺ uptake into the organelle. Identical mt-cAMP dependence of mitochondrial Ca²⁺ uptake was also observed in HeLa cells. Importantly, the enhancing effect of mt-cAMP on Ca²⁺ uptake was independent from both the mitochondrial membrane potential and Ca²⁺ efflux, but was reduced by Epac1 (also known as RAPGEF3) blockade both in intact and in permeabilized cells. Finally, overexpression of sAC in the mitochondrial matrix potentiated aldosterone production implying that the observed positive feedback mechanism of mt-cAMP on mitochondrial Ca²⁺ accumulation may have a role in the rapid initiation of steroidogenesis.

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