Mobile dispersed genetic elements and other middle repetitive DNA sequences in the genomes of Drosophila and mouse: transcription and biological significance
GP Georgiev, YV Ilyin… - Cold Spring Harbor …, 1981 - symposium.cshlp.org
GP Georgiev, YV Ilyin, VG Chmeliauskaite, AP Ryskov, DA Kramerov, KG Skryabin…
Cold Spring Harbor symposia on quantitative biology, 1981•symposium.cshlp.orgFigure 2. Hybridization of cytoplasmic poly (A) § RNA to the fragments of mdg-1 and mdg-3.
Poly (A) § RNA from the D. melanogaster culture ceils labeled with [~ 2p] orthophosphate for
15 hr was obtained as described by Tchurikov et al.(1978). For hybridization of mdg-1, only
the heavy fraction (> 20S) was used. The Southern filters contained the following, mdg-
1:(ad) p58 DNA;(e) EcoRI fragment B; 00 EcoRI fragment C. mdg-3:(gm) p38 DNA. In c,
fragment D is located above A since it is linked to a pBR322 DNA.
Poly (A) § RNA from the D. melanogaster culture ceils labeled with [~ 2p] orthophosphate for
15 hr was obtained as described by Tchurikov et al.(1978). For hybridization of mdg-1, only
the heavy fraction (> 20S) was used. The Southern filters contained the following, mdg-
1:(ad) p58 DNA;(e) EcoRI fragment B; 00 EcoRI fragment C. mdg-3:(gm) p38 DNA. In c,
fragment D is located above A since it is linked to a pBR322 DNA.
Figure 2. Hybridization of cytoplasmic poly (A) § RNA to the fragments of mdg-1 and mdg-3. Poly (A) § RNA from the D. melanogaster culture ceils labeled with [~ 2p] orthophosphate for 15 hr was obtained as described by Tchurikov et al.(1978). For hybridization of mdg-1, only the heavy fraction (> 20S) was used. The Southern filters contained the following, mdg-1:(ad) p58 DNA;(e) EcoRI fragment B; 00 EcoRI fragment C. mdg-3:(gm) p38 DNA. In c, fragment D is located above A since it is linked to a pBR322 DNA.
symposium.cshlp.org
以上显示的是最相近的搜索结果。 查看全部搜索结果