[HTML][HTML] Modulation of N-cadherin junctions and their role as epicenters of differentiation-specific actin regulation in the developing lens

M Leonard, L Zhang, N Zhai, A Cader, Y Chan… - Developmental …, 2011 - Elsevier
M Leonard, L Zhang, N Zhai, A Cader, Y Chan, RB Nowak, VM Fowler, AS Menko
Developmental biology, 2011Elsevier
Extensive elongation of lens fiber cells is a central feature of lens morphogenesis. Our study
investigates the role of N-cadherin junctions in this process in vivo. We investigate both the
molecular players involved in N-cadherin junctional maturation and the subsequent function
of these junctions as epicenters for the assembly of an actin cytoskeleton that drives
morphogenesis. We present the first evidence of nascent cadherin junctions in vivo, and
show that they are a prominent feature along lateral interfaces of undifferentiated lens …
Extensive elongation of lens fiber cells is a central feature of lens morphogenesis. Our study investigates the role of N-cadherin junctions in this process in vivo. We investigate both the molecular players involved in N-cadherin junctional maturation and the subsequent function of these junctions as epicenters for the assembly of an actin cytoskeleton that drives morphogenesis. We present the first evidence of nascent cadherin junctions in vivo, and show that they are a prominent feature along lateral interfaces of undifferentiated lens epithelial cells. Maturation of these N-cadherin junctions, required for lens cell differentiation, preceded organization of a cortical actin cytoskeleton along the cells' lateral borders, but was linked to recruitment of α-catenin and dephosphorylation of N-cadherin-linked β-catenin. Biochemical analysis revealed differentiation-specific recruitment of actin regulators cortactin and Arp3 to maturing N-cadherin junctions of differentiating cells, linking N-cadherin junctional maturation with actin cytoskeletal assembly during fiber cell elongation. Blocking formation of mature N-cadherin junctions led to reduced association of α-catenin with N-cadherin, prevented organization of actin along lateral borders of differentiating lens fiber cells and blocked their elongation. These studies provide a molecular link between N-cadherin junctions and the organization of an actin cytoskeleton that governs lens fiber cell morphogenesis in vivo.
Elsevier
以上显示的是最相近的搜索结果。 查看全部搜索结果