Mycoplasmosis is the most important respiratory disease poses a serious threat to the small ruminant population by causing heavy economic losses in Northern and Southern regions of the country (Saddique et al., 2012; Samiullah, 2013; Banaras et al., 2016). Among the different pathogenic species of mycoplasma, contagious caprine pleuropneumonia (CCPP) is highly fetal disease caused by Mycoplasma capricolum sub specie capripneumoniae (Mccp)(OIE, 2012). The CCPP was first time reported in Algeria in 1873 and later in many countries of Africa, Europe, Middle East and Asia (Tigga et al., 2014; Atim et al., 2016). In Pakistan the disease was considered to be caused by Mycoplasma mycoides sub specie capri till the molecular confirmation as Mccp in Baluchistan by Awan et al.(2010). Recently in the international collaborative study Mccp was confirmed in the northern Pakistan and Tajikistan (Peyraud et al., 2014). The disease also prevalent in different areas and livestock research centers of Punjab, Pakistan (Shahzad et al., 2016). The disease is widely spread in many countries of the globe and considered to be caused by six different pathogenic species called as Mycoplasma cluster. However, the classical form of disease is caused by