Molecular and biochemical characterization of novel glucokinases from Trypanosoma cruzi and Leishmania spp.
AJ Cáceres, W Quiñones, M Gualdrón… - Molecular and …, 2007 - Elsevier
Glucokinase genes, found in the genome databases of Trypanosoma cruzi and Leishmania
major, were cloned and sequenced. Their expression in Escherichia coli resulted in the
synthesis of soluble and active enzymes, TcGlcK and LmjGlcK, with a molecular mass of
43kDa and 46kDa, respectively. The enzymes were purified, and values of their kinetic
parameters determined. The Km values for glucose were 1.0 mM for TcGlcK and 3.3 mM for
LmjGlcK. For ATP, the Km values were 0.36 mM (TcGlcK) and 0.35 mM (LmjGlcK). A lower …
major, were cloned and sequenced. Their expression in Escherichia coli resulted in the
synthesis of soluble and active enzymes, TcGlcK and LmjGlcK, with a molecular mass of
43kDa and 46kDa, respectively. The enzymes were purified, and values of their kinetic
parameters determined. The Km values for glucose were 1.0 mM for TcGlcK and 3.3 mM for
LmjGlcK. For ATP, the Km values were 0.36 mM (TcGlcK) and 0.35 mM (LmjGlcK). A lower …
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