Anaplasma marginale in the bovine (cattle and buffaloes) and Rhipicephalus (Boophilus)
microplus tick belonged to the tribal area of coastal South Gujarat, India, by amplifying 576
bp of major surface protein (msp) 5 gene using custom designed primers in the polymerase
chain reaction (PCR). Results The PCR detection limit was up to 20 parasites/µl of blood in
sensitivity experiment, and observed 100% specificity against Trypanosoma evansi, Babesia …