Molecular cloning and functional characterization of brefeldin A-ADP-ribosylated substrate: a novel protein involved in the maintenance of the Golgi structure
Brefeldin A (BFA) is a fungal metabolite that disassembles the Golgi apparatus into tubular
networks and causes the dissociation of coatomer proteins from Golgi membranes. We have
previously shown that an additional effect of BFA is to stimulate the ADP-ribosylation of two
cytosolic proteins of 38 and 50 kDa (brefeldin A-ADP-riboslyated substrate (BARS)) and that
this effect greatly facilitates the Golgi-disassembling activity of the toxin. In this study, BARS
has been purified from rat brain cytosol and microsequenced, and the BARS cDNA has …
networks and causes the dissociation of coatomer proteins from Golgi membranes. We have
previously shown that an additional effect of BFA is to stimulate the ADP-ribosylation of two
cytosolic proteins of 38 and 50 kDa (brefeldin A-ADP-riboslyated substrate (BARS)) and that
this effect greatly facilitates the Golgi-disassembling activity of the toxin. In this study, BARS
has been purified from rat brain cytosol and microsequenced, and the BARS cDNA has …
[引用][C] Molecular cloning and functional characterization of brefeldin A-ADP-ribosylated substrate. A novel protein involved in the maintenance of the Golgi structure.
Page 31854, under “GST Pulldown Assay”: In the seventh line from the bottom of that
paragraph, the composition of a buffer (NETN) is given as “(0.01% Nonidet P-40, 20 mM Tris-
HCl (pH 8.8), 1 mM EDTA, and 40 mM NaCl.” It should read “(0.01% Nonidet P-40, 20 mM
Tris-HCl (pH 8.0), 1 mM EDTA, and 40 mM NaCl).”
paragraph, the composition of a buffer (NETN) is given as “(0.01% Nonidet P-40, 20 mM Tris-
HCl (pH 8.8), 1 mM EDTA, and 40 mM NaCl.” It should read “(0.01% Nonidet P-40, 20 mM
Tris-HCl (pH 8.0), 1 mM EDTA, and 40 mM NaCl).”
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