[HTML][HTML] Molecular detection and dense granule antigen 6 genotyping of feline Toxoplasma gondii in Phayao, Thailand

C Suwancharoen, C Phuangsri, K Prakhammin… - Veterinary …, 2022 - ncbi.nlm.nih.gov
Materials and Methods: A total of 124 fecal samples were collected from owned cats in the
Muang district, Phayao, Thailand, from January to December 2020. Fecal samples were
tested for the presence of T. gondii DNA using targeted B1 gene polymerase chain reaction
(PCR) amplification, and positive samples were subsequently analyzed for their T. gondii
genotype through PCR-restriction fragment length polymorphism (RFLP) analysis and
sequencing of the GRA6 gene. Results: Among the 124 samples, 46 (37.1%) were tested …

Molecular detection and dense granule antigen 6 genotyping of feline Toxoplasma gondii in Phayao, Thailand, Veterinary World, 15 (9): 2309–2314

C Suwancharoen, C Phuangsri, K Prakhammin, O Japa - 2022 - veterinaryworld.org
Abstract Background and Aim: Globally, toxoplasmosis is an important zoonotic parasite
infection of many warm-blooded animals (including humans). Toxoplasma gondii oocysts
are widespread, and their contamination can be primarily attributed to the members of the
Felidae family. This study aimed to estimate the prevalence and determine the dense
granule antigen 6 (GRA6) genotype of T. gondii among domestic cats in the Phayao
Province, Thailand. Materials and Methods: A total of 124 fecal samples were collected from …
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