Monitoring Glycolysis and Respiration Highlights Metabolic Inflexibility of Cryptococcus neoformans
S Lev, C Li, D Desmarini, D Liuwantara, TC Sorrell… - Pathogens, 2020 - mdpi.com
Cryptococcus neoformans is a human fungal pathogen that adapts its metabolism to cope
with limited oxygen availability, nutrient deprivation and host phagocytes. To gain insight
into cryptococcal metabolism, we optimized a protocol for the Seahorse Analyzer, which
measures extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) as
indications of glycolytic and respiratory activities. In doing so we achieved effective
immobilization of encapsulated cryptococci, established Rotenone/Antimycin A and 2 …
with limited oxygen availability, nutrient deprivation and host phagocytes. To gain insight
into cryptococcal metabolism, we optimized a protocol for the Seahorse Analyzer, which
measures extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) as
indications of glycolytic and respiratory activities. In doing so we achieved effective
immobilization of encapsulated cryptococci, established Rotenone/Antimycin A and 2 …
[PDF][PDF] Monitoring Glycolysis and Respiration Highlights Metabolic Inflexibility of Cryptococcus neoformans
TC Sorrell, WJ Hawthorne, JT Djordjevic - academia.edu
Cryptococcus neoformans is a human fungal pathogen that adapts its metabolism to cope
with limited oxygen availability, nutrient deprivation and host phagocytes. To gain insight
into cryptococcal metabolism, we optimized a protocol for the Seahorse Analyzer, which
measures extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) as
indications of glycolytic and respiratory activities. In doing so we achieved effective
immobilization of encapsulated cryptococci, established Rotenone/Antimycin A and 2 …
with limited oxygen availability, nutrient deprivation and host phagocytes. To gain insight
into cryptococcal metabolism, we optimized a protocol for the Seahorse Analyzer, which
measures extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) as
indications of glycolytic and respiratory activities. In doing so we achieved effective
immobilization of encapsulated cryptococci, established Rotenone/Antimycin A and 2 …
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