Multilocus sequence typing of Salmonella strains by high-throughput sequencing of selectively amplified target genes

P Singh, SL Foley, R Nayak, YM Kwon - Journal of microbiological methods, 2012 - Elsevier
Rapid development of next generation sequencing (NGS) technologies in recent years has
made whole genome sequencing of bacterial genomes widely accessible. However, it is
often unnecessary or not feasible to sequence the whole genome for most applications of
genetic analyses in bacteria. Selectively capturing defined genomic regions followed by
NGS analysis could be a promising approach for high-resolution molecular typing of a large
set of strains. In this study, we describe a novel and straightforward PCR-based target …
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