Multiple discontinuous ligand-mimetic antibody binding sites define a ligand binding pocket in integrin αIIbβ3
W Puzon-McLaughlin, T Kamata, Y Takada - Journal of Biological …, 2000 - ASBMB
Integrin α IIb β 3, a platelet fibrinogen receptor, is critically involved in thrombosis and
hemostasis. However, how ligands interact with α IIb β 3 has been controversial. Ligand-
mimetic anti-α IIb β 3 antibodies (PAC-1, LJ-CP3, and OP-G2) contain the RGD-like RYD
sequence in their CDR3 in the heavy chain and have structural and functional similarities to
native ligands. We have located binding sites for ligand-mimetic antibodies in α IIb and β 3
using human-to-mouse chimeras, which we expect to maintain functional integrity of α IIb β …
hemostasis. However, how ligands interact with α IIb β 3 has been controversial. Ligand-
mimetic anti-α IIb β 3 antibodies (PAC-1, LJ-CP3, and OP-G2) contain the RGD-like RYD
sequence in their CDR3 in the heavy chain and have structural and functional similarities to
native ligands. We have located binding sites for ligand-mimetic antibodies in α IIb and β 3
using human-to-mouse chimeras, which we expect to maintain functional integrity of α IIb β …