Multiplexed bioluminescence microscopy via phasor analysis

Z Yao, CK Brennan, L Scipioni, H Chen, KK Ng… - Nature …, 2022 - nature.com
… need for more effective multiplexing at the microscale by developing bioluminescent phasor,
a … sensitivity and broad dynamic range of BLI with the resolving power of phasor imaging. …

[PDF][PDF] Multiplexed bioluminescence microscopy via phasor analysis

CK Brennan, Z Yao, L Scipioni, H Chen, K Ng… - Biophysical …, 2022 - cell.com
Bioluminescent phasor enabled rapid discrimination of multiple … Furthermore, we were
able to perform multiplexed, continuous … The merger of bioluminescence and phasor analysis

Monitoring macrophage polarization with gene expression reporters and bioluminescence phasor analysis

G Tedeschi, MX Navarro, L Scipioni… - … & Biomedical Imaging, 2024 - ACS Publications
multiplexed imaging in complex 3D environments while maintaining cellular resolution. We
aimed to fill this void using bioluminescent … -cell bioluminescent readouts of multiplexed gene …

Multiplexed near infrared fluorescence lifetime imaging in turbid media

M Harel, U Arbiv, R Ankri - Journal of Biomedical Optics, 2024 - spiedigitallibrary.org
… Applying phasor analyses, we enable the multiplexing of distinct targets within a single
FLI image. Leveraging the state-of-the-art single-photon avalanche diode (SPAD) time-gated …

Advancements in phasor-based FLIM: multi-component analysis and lifetime probes in biological imaging

D Li, X Liu, F Dong, W Li - Journal of Materials Chemistry B, 2025 - pubs.rsc.org
… Multi-component analysis of the phasor plot enables multiplexed detection, such as using
precise differentiation of six highly overlapping bioluminescent probes in live cells. Additionally…

Multiplexed bioluminescence imaging with a substrate unmixing platform

CK Brennan, Z Yao, AA Ionkina, CM Rathbun… - Cell chemical …, 2022 - cell.com
… , and multiplexed bioluminescent readouts, we developed an analysis pipeline featuring …
A MATLAB algorithm was written to analyze bioluminescent images generated from the …

Three-dimensional deep tissue multiphoton frequency-domain fluorescence lifetime imaging microscopy via phase multiplexing and adaptive optics

Y Zhang, IH Guldner, EL Nichols… - … Microscopy in the …, 2019 - spiedigitallibrary.org
… , in a multiplexing manner; hence we call this technique phase multiplexing FLIM. The PM-…
By drawing region-of-interests (ROIs) on phasor plots using our custom-written program, the …

Rapid, multiplexed bioluminescence imaging at all length scales

CK Brennan - 2022 - escholarship.org
multiplexed bioluminescence imaging at the microscale. This work merged BLI with phasor
analysis, … that can be applied to bioluminescent phasor analysis. These probes were applied …

Creating coveted bioluminescence colors for simultaneous multi-color bioimaging

M Hattori, T Wazawa, M Orioka, Y Hiruta, T Nagai - Science Advances, 2025 - science.org
… Yao et al. developed a phasor analysis method that is commonly used to distinguish … detect
multiple bioluminescence colors in a single shot, enabling enormous multiplex color imaging …

Preprint Highlight: Multiplexed bioluminescence microscopy via phasor analysis

D Takao - Molecular Biology of the Cell, 2022 - Am Soc Cell Biol
multiplexing of bioluminescence imaging, this study applies phasor analysis that can cluster
profiles, in the phasor … to specific bioluminescence probes. The authors demonstrate that …