… Encouraged by the similarity of the trajectories between the traditional and NMR-guided
evolution, we undertook a prospective study to determine whether CSP analysis could be used …

… evolution methodologies17-25, thus we set up to explore whether an NMR-guided approach
can be successfully used to evolve … a starting point for the evolution. For the unbiased test of …

… In a further test of the NMR-guided approach we applied it to … residues identified in NMR-guided
saturation mutagenesis … starting point for the directed evolution experiment. This level of …

… (29-31) Despite previous attempts to improve enzyme tolerance to ILs via directed evolution,
(32-34) this work represents, to the best of our knowledge, the first attempt to rationally …

… rounds of evolution. NMR-guided evolution strategy eliminates excessive mutagenesis
and has the potential to unlock exciting avenues in directed evolution of protein catalysts. …

… more sophisticated and effective directed evolution techniques have been developed6, and
in this paper we have shown how this new NMR-guided approach allowed the evolution of a …

… Likewise, the MolProbity scoring for the NMR-guided structure of 2 is much improved over
the CYANA structure. These improvements in the structures of our peptide catalysts likely …

… (function terminates if every possible combination of direct neighbors of at … 1.6 s, delay for
the evolution of nJCH or of MS fragmentations to the … 2.0 s, delay for the evolution of 1JCH D4 …

… productive mutations to improve the output of directed evolution. We have found a strong …
The combination of three mutations identified by NMR-guided directed evolution resulted in …

… Recent work19 has suggested that catalytic hotspots for focusing directed evolution experiments
can be … Our goal in this work is to use NMR-guided directed evolution combined with …