BACKGROUND: The use of molecular methods for rotavirus characterisation provides
increased sensitivity for typing, and allows the identification of putative reassortant strains.
However, due to the constant accumulation of point mutations through genetic drift; and to
the emergence of novel genotypes; and possibly zoonotic transmission and subsequent
reassortment, the reagents and methods used for genotyping require close monitoring and
updating. OBJECTIVES: To design and evaluate a new VP4 consensus oligonucleotide …