Metabolic Syndrome (MS) may be characterized with obesity and insulin resistance. In obesity, the increased accumulation of fat in the circulation and tissues will lead to Reactive Oxygen Species (ROS), followed by oxidative stress. Nonalcoholic Fatty Liver Disease (NAFLD), the accumulation of excess free fat in the form of triglycerides caused by lipolysis due to insulin resistance, can cause inflammation and liver cells damage characterized by increased serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT). Nigella Sativa (Black Seed) oil is known to be efficacious as an antioxidant that can improve hepatic function. This study uses a cross over design to determine the effect of black seed oil with a dose of 1.5 ml/day and 3 ml/day on SGOT and SGPT activities in patients with SM. A total of 62 SM patients were divided into two groups which received 1.5 ml/day and 3 ml/day respectively for 20 days, and after 7-day washout, the doses were reversed. Measurements were performed on the 21st and 49th days. The results showed a significant difference in SGOT activity decrease in patients receiving 1.5 ml/day compared with those receiving 3 ml/day. However, in terms of SGPT, there was no significant difference between the two treatment groups.