This paper presents a novel strategy for determination of the illegal dye Sudan I in paprika
powder. The method is based on fluorescence spectroscopy combined with second-order
calibration, which was employed for the first time for this purpose. The method is non-
destructive and requires no sample preparation. It was probed that Sudan I exhibited
fluorescence; however, the color of paprika samples affected the signal and it was not
possible to quantify this adulterant by means of univariate and first-order calibration. To …

Design). The two parameters varied were ASTA color values and Sudan I concentration.
ASTA 125 color values of paprika powder varied between 25 and 150 based on selected
paprika samples and 126 the samples were spiked with Sudan I dye at several
concentrations, between 0.27 and 24 mg/g. 127