Surface plasmon resonance (SPR) is a widely used, affinity based, label-free biophysical
technique to investigate biomolecular interactions. The extraction of rate constants requires
accurate identification of the particular binding model. The bivalent analyte model involves
coupled non-linear differential equations. No clear procedure to identify the bivalent analyte
mechanism has been established. In this report, we propose a unique signature for the
bivalent analyte model. This signature can be used to distinguish the bivalent analyte model …