Nucleic acid detection with CRISPR-Cas13a/C2c2

JS Gootenberg, OO Abudayyeh, JW Lee… - Science, 2017 - science.org
… Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen …
To simulate sample detection without nucleic acid purification, we measured detection of …

N 1-Methyladenosine detection with CRISPR-Cas13a/C2c2

Y Chen, S Yang, S Peng, W Li, F Wu, Q Yao… - Chemical …, 2019 - pubs.rsc.org
… In summary, the CRSIPR-Cas13a system exhibited limitless application in nucleic acid
detection. In this work, we first extended this system to the detection of epigenetic modification. In …

[PDF][PDF] Nucleic acid detection with CRISPR-Cas13a/C2c2

AJ Dy, J Joung, V Verdine, N Donghia, NM Daringer… - intbio.org
nucleic acid detection platform with attomolar sensitivity based on … To simulate sample
detection without nucleic acid purification, we measured detection of ssRNA 1 spiked into human …

[HTML][HTML] Design of crRNA to regulate MicroRNAs related to metastasis in colorectal cancer using CRISPR-C2c2 (Cas13a) technique

ST Houseini, F Nemati, A Sattari, M Azadeh… - Cell Journal …, 2023 - ncbi.nlm.nih.gov
Colorectal cancer (CRC) is the third most prevalent cancer with the second-highest mortality
rate worldwide. microRNAs (miRNAs) of cancer-derived exosomes have shown promising …

A rapid and efficient platform for antiviral crRNA screening using CRISPR-Cas13a-based nucleic acid detection

L Yang, Y Zhang, W Yi, X Dong, M Niu, Y Song… - Frontiers in …, 2023 - frontiersin.org
… In this study, we established the CRISPR-Cas13a nucleic acid detection based on the
same principle as the published articles. And nucleic acid detection and antiviral technology …

Rapid detection of genotype II African swine fever virus using CRISPR Cas13a-based lateral flow strip

N Wei, B Zheng, J Niu, T Chen, J Ye, Y Si, S Cao - Viruses, 2022 - mdpi.com
… The viral capsid protein p72, encoded by the B646L gene, is highly conserved and well
characterized, making it a widely used target for both nucleic acid detection and phylogenetic …

A Sensitive Nucleic Acid Detection Platform for Foodborne Pathogens Based on CRISPR-Cas13a System Combined with Polymerase Chain Reaction

R Liu, S Ali, D Huang, Y Zhang, P Lü, Q Chen - Food Analytical Methods, 2023 - Springer
… a nucleic acid detection platform for sensitive detection of foodborne pathogens. Staphylococcus
aureus (ATCC® 29213™) as a model pathogen was used to evaluate the detection

Nucleic Acid Amplification-Free Digital Detection Method for SARS-CoV-2 RNA Based on Droplet Microfluidics and CRISPR–Cas13a

X Shan, F Gong, Y Yang, J Qian, Z Tan, S Tian… - Analytical …, 2023 - ACS Publications
… (2) Reverse transcription quantitative polymerase chain reaction (RT-qPCR), the “gold
standard method” for nucleic acid detection with the advantages of high sensitivity and specificity, …

Computational Analysis of crRNA to Regulate hsa-miR-301b-3p and hsa-miR-21 related to metastasis and cell proliferation in acute myeloid leukemia (AML) using …

O Moeini, A Gholamzad, M Khatibi, M Nourikhani… - 2023 - researchsquare.com
… So far, this type of CRISPR-based RNA targeting tool has already been used in biomedical
applications, such as the detection of specific sequences of patient viral RNA or tumor …

CRISPR/Cas13a induced exponential amplification for highly sensitive and specific detection of circular RNA

P Song, P Zhang, K Qin, F Su, K Gao, X Liu, Z Li - Talanta, 2022 - Elsevier
… of all sequence-specific RNA targets, indicating that our CRISPR/Cas13a induced
exponential amplification assay has great potential for the detection of RNA biomarkers in both …