Oligomerization state-dependent activation of NF-κB signaling pathway by adipocyte complement-related protein of 30 kDa (Acrp30)
Adipocyte complement-related protein of 30 kDa (Acrp30)/adiponectin is an adipocyte-
derived hormone that affects lipid and glucose metabolism in muscle and liver, but its
physical and biochemical properties are poorly characterized. Here we have used several
approaches to show that Acrp30 expressed in and purified fromEscherichia coli and human
embryonic kidney 293T cells forms trimers and hexamers; 293T cells also produce a higher
molecular weight species. Similar Acrp30 oligomers were found in mouse serum as well as …
derived hormone that affects lipid and glucose metabolism in muscle and liver, but its
physical and biochemical properties are poorly characterized. Here we have used several
approaches to show that Acrp30 expressed in and purified fromEscherichia coli and human
embryonic kidney 293T cells forms trimers and hexamers; 293T cells also produce a higher
molecular weight species. Similar Acrp30 oligomers were found in mouse serum as well as …