[HTML][HTML] Optimal timing for Agrobacterium-mediated DNA transformation of Trichoderma reesei conidia revealed by live cell imaging
S Kilaru, M Schuster, R Murray, G Steinberg - Fungal Genetics and Biology, 2020 - Elsevier
Trichoderma reesei is the foremost fungal producer of enzymes for industrial processes.
Here, we use fluorescent live cell imaging of germinating conidia to improve Agrobacterium
tumefaciens-mediated transformation (ATMT) efficiency. We define the timing of (a)
morphological changes and (b) nuclear reorganisation during initial conidia germination.
This reveals that conidia swell for 7 h, during which nuclei undergo 2 non-synchronised
mitotic divisions. Histones are recruited to the nucleus during the first 2 h, suggesting that …
Here, we use fluorescent live cell imaging of germinating conidia to improve Agrobacterium
tumefaciens-mediated transformation (ATMT) efficiency. We define the timing of (a)
morphological changes and (b) nuclear reorganisation during initial conidia germination.
This reveals that conidia swell for 7 h, during which nuclei undergo 2 non-synchronised
mitotic divisions. Histones are recruited to the nucleus during the first 2 h, suggesting that …