Optimization of 90Y-antiCD20 preparation for radioimmunotherapy
N Gholipour, A Vakili, E Radfar… - Journal of cancer …, 2013 - journals.lww.com
Aims: A further tried and tested improvement to the unmodified antibody has been its
coupling to the beta-emitters Y-90. The optimization of 90 Y-antiCD20
radioimmunoconjugate production and quality control methods for future clinical studies in
the country was targeted in this work. Materials and Methods: The antibody was labeled with
90 Y-yttrium chloride (185 MBq) after conjugation with freshly prepared ccDTPA. Y-90
chloride was obtained by thermal neutron flux (4× 10 13 n/cm 2/s) of a natural Y 2 O 3 …
coupling to the beta-emitters Y-90. The optimization of 90 Y-antiCD20
radioimmunoconjugate production and quality control methods for future clinical studies in
the country was targeted in this work. Materials and Methods: The antibody was labeled with
90 Y-yttrium chloride (185 MBq) after conjugation with freshly prepared ccDTPA. Y-90
chloride was obtained by thermal neutron flux (4× 10 13 n/cm 2/s) of a natural Y 2 O 3 …
Optimization of^ sup 90^ Y-antiCD20 preparation for radioimmunotherapy
A Vakili, A Jalilian, E Radfar… - Iranian Journal of …, 2010 - search.proquest.com
Methods: The antibody was labeled with 90Y-yttrium chloride (185 MBq) after conjugation
with freshly prepared ccDTPA. Y-90 chloride was obtained by thermal neutron flux (4× 1013
n. cm-2. s-1) of a natural Y2O3 sample, dissolved in acidic media. Radiolabeling was
completed in 24 hours by the addition of DTPArituximab conjugate at room temperature.
Results: Radiochemical purity of 96%(using ITLC) was obtained for final
radioimmunoconjugate (Specific activity= 440-480 MBq/mg). The final isotonic 90Y …
with freshly prepared ccDTPA. Y-90 chloride was obtained by thermal neutron flux (4× 1013
n. cm-2. s-1) of a natural Y2O3 sample, dissolved in acidic media. Radiolabeling was
completed in 24 hours by the addition of DTPArituximab conjugate at room temperature.
Results: Radiochemical purity of 96%(using ITLC) was obtained for final
radioimmunoconjugate (Specific activity= 440-480 MBq/mg). The final isotonic 90Y …