Optimization of the oligonucleotide ligation assay for the detection of nevirapine resistance mutations in Zimbabwean Human Immunodeficiency Virus type-1 subtype …
J Mutsvangwa, IA Beck, L Gwanzura… - Journal of virological …, 2014 - Elsevier
An oligonucleotide ligation assay (OLA) designed to detect Human Immunodeficiency Virus
type-1 (HIV)-drug-resistance to the nevirapine (NVP) selected mutations K103N, Y181C,
V106M and G190A was used to evaluate 200 archived dried blood spots (DBS) from
infected infants participating in the Zimbabwean Early Infant Diagnosis (EID) Program.
Consensus sequencing of specimens with indeterminate OLA results was performed to
identify genetic sequence polymorphisms that appeared to compromise performance of the …
type-1 (HIV)-drug-resistance to the nevirapine (NVP) selected mutations K103N, Y181C,
V106M and G190A was used to evaluate 200 archived dried blood spots (DBS) from
infected infants participating in the Zimbabwean Early Infant Diagnosis (EID) Program.
Consensus sequencing of specimens with indeterminate OLA results was performed to
identify genetic sequence polymorphisms that appeared to compromise performance of the …
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