[HTML][HTML] Optimized dCas9 programmable transcriptional activators for plants
MH Zinselmeier, JA Casas‐Mollano… - Plant Biotechnology …, 2024 - pmc.ncbi.nlm.nih.gov
… We also added plant‐evolved AD sequences from plant pathogen effector proteins such
as TALE proteins from Xanthomonas, along with plant‐derived sequences from
transcription preinitiation complexes such as 14‐3‐3 scaffolding proteins. The ability of
these ADs to function in the context of a dCas9‐based PTA was tested in protoplasts from
monocot and dicot species (Tables S2 and S3, Figures S1–S7). Specifically, we confirmed
that in the Arabidopsis protoplast assay, the SunTag‐PTA, in which ADs are recruited to …
as TALE proteins from Xanthomonas, along with plant‐derived sequences from
transcription preinitiation complexes such as 14‐3‐3 scaffolding proteins. The ability of
these ADs to function in the context of a dCas9‐based PTA was tested in protoplasts from
monocot and dicot species (Tables S2 and S3, Figures S1–S7). Specifically, we confirmed
that in the Arabidopsis protoplast assay, the SunTag‐PTA, in which ADs are recruited to …
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