Over-production of human interferon-γ by HCDC of recombinant Escherichia coli
A simple fed-batch process was developed using a modified variable specific growth rate
feeding strategy for high cell density cultivation of Escherichia coli BL21 (DE3) expressing
human interferon-gamma (hIFN-γ). The feeding rate was adjusted to achieve the maximum
attainable specific growth rate during fed-batch cultivation. In this method, specific growth
rate was changed from a maximum value of 0.55 h− 1 at the beginning of feeding and then it
was reduced to 0.4 h− 1 at induction time. The final concentration of biomass and IFN-γ was …
feeding strategy for high cell density cultivation of Escherichia coli BL21 (DE3) expressing
human interferon-gamma (hIFN-γ). The feeding rate was adjusted to achieve the maximum
attainable specific growth rate during fed-batch cultivation. In this method, specific growth
rate was changed from a maximum value of 0.55 h− 1 at the beginning of feeding and then it
was reduced to 0.4 h− 1 at induction time. The final concentration of biomass and IFN-γ was …
以上显示的是最相近的搜索结果。 查看全部搜索结果