PD36-06 EFFICACY OF ROLIPRAM LOADED NANOSPHERES IN LOCALIZED DELIVERY TO SITES OF NERVE INJURY FOR PREVENTION OF POST …

N Patel, AH Salmasi, M Dinizo, I Faiena… - The Journal of …, 2015 - auajournals.org
The Journal of Urology, 2015auajournals.org
METHODS Rolipram loaded NSP which are tyrosine-derived copolymers that
spontaneously self-assemble in aqueous media were produced. Twenty-four male Sprague
Dawley rats were randomized into 4 groups. Three groups of rats received bilateral crush
nerve injury (BCNI) and one group received no injury (sham, n= 6). Of the rats that
underwent BCNI, one group received no intervention (BNCI, n= 6), while one group received
rolipram loaded NSP over site of induced crush injury bilaterally (BCNI+ Rolipram+ NSP, n …
METHODS
Rolipram loaded NSP which are tyrosine-derived copolymers that spontaneously self-assemble in aqueous media were produced. Twenty-four male Sprague Dawley rats were randomized into 4 groups. Three groups of rats received bilateral crush nerve injury (BCNI) and one group received no injury (sham, n= 6). Of the rats that underwent BCNI, one group received no intervention (BNCI, n= 6), while one group received rolipram loaded NSP over site of induced crush injury bilaterally (BCNI+ Rolipram+ NSP, n= 6), and the last group was treated with empty nanospheres after BCNI (NSP, n= 6). After 14 days, each rat underwent erectile function testing and immunohistochemistry of phalloidin, neurofilaments, and neuronal nitric oxide synthase in the penile dorsal nerve was performed on mid penile cross-sections.
RESULTS
Erectile function in rats treated with Rolipram loaded NSPs was significantly improved compared to the BNCI group (p< 0.05). Phalloidin, neurofilament, and neuronal nitric oxide synthase quantified expression was significantly greater in Rolipram loaded NSP group compared to the BNCI group (p< 0.05, Figure 1).
CONCLUSIONS
Immediate placement of a localized drug delivery system such as Rolipram loaded NSPs to the site of nerve injury showed improvement in erectile function. This therapeutic approach of localized drug delivery at the time of surgery would benefit a large number of post-RP patients and improve cavernous nerve regeneration.
auajournals.org

Google学术搜索按钮

example.edu/paper.pdf
搜索
获取 PDF 文件
引用
References