Paraffin immunofluorescence is a well established “salvage” technique in renal pathology
when representative glomeruli are not found in the fresh frozen tissue sent for routine direct
immunofluorescence studies. A step of enzymatic digestion of the formalin-fixed paraffin-
embedded biopsy exposes the antigenic immune complexes and allows staining with
fluorochrome-tagged antibodies. We explored the utility of the technique of paraffin
immunofluorescence outside the kidney in certain specific scenarios including extra renal …