[PDF][PDF] Physiological and pathological effects of diminazene on pulmonary hypertension: a controlled rat model study

A Sengul, S Arkan, C Vural, C Ozer… - … OF CLINICAL AND …, 2016 - e-century.us
A Sengul, S Arkan, C Vural, C Ozer, BY Bayrak, A Tas, ND Kocak, UA Akaturk
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, 2016e-century.us
Objectives: To evaluate the effects of diminazene (DMZ) on monocrotaline (MCT)-induced
pulmonary arterial hypertension (PAH). Methods: In total, 32 nine-week-old male Wistar-
Albino rats (170-240 g) were divided into three groups: control (n= 10), PAH (n= 15), and
PAH+ DMZ groups (n= 7). On the first day, 60 mg/kg MCT was injected intraperitoneally in
the PAH and PAH+ DMZ groups. On the 21st day, 15 mg/kg/day DMZ was injected, and the
animals were followed for 35 days. On the 35th day, the exercise capacity of rats was …
Abstract
Objectives: To evaluate the effects of diminazene (DMZ) on monocrotaline (MCT)-induced pulmonary arterial hypertension (PAH). Methods: In total, 32 nine-week-old male Wistar-Albino rats (170-240 g) were divided into three groups: control (n= 10), PAH (n= 15), and PAH+ DMZ groups (n= 7). On the first day, 60 mg/kg MCT was injected intraperitoneally in the PAH and PAH+ DMZ groups. On the 21st day, 15 mg/kg/day DMZ was injected, and the animals were followed for 35 days. On the 35th day, the exercise capacity of rats was analyzed through a modified forced swimming test. After measuring right ventricular systolic pressure using an open-chest method, right ventricle hypertrophy and pulmonary vascular remodeling were evaluated histopathologically. Results: On the 35th day, the mortality rate was zero in the control group, 53.1% in the PAH group, and 14.3% in the PAH+ DMZ group. A significant decrease was observed in mortality rates with DMZ, and significant recovery was noted in median life spans (P= 0.16 and P= 0.01, respectively). DMZ had no significant effect on exercise capacity, right ventricle hypertrophy, or right ventricle systolic pressure, whereas there was significant recovery in pulmonary artery muscular layer thickness (P= 0.046). Conclusions: DMZ prolonged life expectancy in PAH and decreased pulmonary arterial muscularization. Thus, it may be a new candidate treatment for PAH.
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