In Tunisia, the cultivation of the date palm is the pivot of the agricultural activity of the regions of South West and particularly in the areas of Djerid and Nefzaoua. It constitutes the framework of the oases which are unique sources of greenery and life in the middle of the desert. The cultivators of the date palm were able to deduce the importance of the origin of the pollen used on the quantity and the quality of the production of the dates. Consequently, they use selective pollinating male varieties. The importance of male palms, locally named" Dokkars" on the quality and quantity of dates has been the subject of numerous works. In Tunisia, this genetic potential of the date palm, unrecognized wealth is now threatened, which makes it necessary to prospect and study this phylogenetic resource in order to protect it by the selection of better feet. Our present work, which is part of this framework, concerns the study of thirty samples of pollen harvested from the palm groves of the region of Djérid. In a first step, a characterization of the physiological parameters of the pollen was carried out, namely the germination test, viability, pH, conductivity and water content. In the second part, we worked only on ten pollinators. We tried to evaluate the effect of pollen conditioning temperature on the two main fertility parameters (germination and viability) by selecting two temperatures: room temperature and 4 C and this over a period of 8 months. The results obtained showed: the existence of variability in physiological characteristics from one individual to another, which reflects the great diversity of the Dokkars; The existence of a negative correlation between germination rate and pollen conductivity. Finally, the use of the refrigerator at 4 C, allows a better conservation of viability and germination of pollen.