Lung cancer is the leading cause of cancer-related death worldwide, yet in vitro disease
models have been limited to traditional 2D culture utilizing cancer cell lines. In contrast,
recently developed 3D models (organoids) have been adopted by researchers to improve
the physiological relevance of laboratory study. We have hypothesized that 3D hydrogel-
based models will allow for improved disease replication and characterization over standard
2D culture using cells taken directly from patients. Here, we have leveraged the use of 3D …

Lung cancer is the leading cause of cancer-related deaths worldwide, yet most currently
used in vitro cancer models are confined to traditional 2D cell culture conditions. Recently;
however, innovative 3D models such as tumor tissue equivalents (organoids) have been
adopted by researchers to recapitulate tissue architecture and physiology in order to
improve disease modeling and drug testing. We have hypothesized that 3D lung organoids,
incorporating cells directly from patients, will enable personalized disease modeling and …