Low-cost point-of-care (POC) diagnostics play several critical roles in healthcare strategies, particularly for detection, therapy, economy, health management, and surveillance. Any user can now perform rapid, efficient, inexpensive, and easy analysis anywhere at any time and various kind of hybrid biosensing devices improved the POC analyses. This review describes recent developments on the application of various types of portable biosensing devices with focus on smartphone-enabled biosensing in which analysis, diagnostics, and communications are coupled. Further, we provide an overview of recent biosensing platforms, from optical to electrochemical biosensors and consider future opportunities for POC devices for clinical analysis and infectious diseases assessment.