carried out using polyphenol oxidase (PPO) extracted from leaves of three fast growing
plants Parthenium hysterophorus, Alternanthera sessilis and Jatropha curcas. PPO of all
these were characterized for optimum temperature, pH and substrate concentration.
Optimum temperature for all three was 500C and their pH optima lies in acidic range.
Optimum catechol concentration is in between 0.9–1.0 M.