Experimental features for brine shrimp (Artemia urmiana) enrichment with two bacillus species and yeast which isolated from Bluga sturgeon fish (Huso huso) gut have been prepared in laboratory of Gonbad Kavous University (Gonbad kavous-Iran). For present study three treatments with three replicates and three control treatments with three replicate was prepared. Brine shrimp cysts after incubation and isolation from other cysts and cysts sack was enriched with three suspension of Bacillus subtilis (1/4997× 10±0/308× 10 CFU/L), Bacillus licheniformis (1/5017× 10±0/275× 10 CFU/L) 8 7 8 7 and Saccharomyces cerevisiae (1/5057× 10±0/297× 10 CFU/L). This two bacteria and bakery yeast are 7 6 members of probiotics. Enrichment was carried out for 24 hours in four times period: from enrichment starting (t) respectively after 5, 10 and 24 hours sampling have been done and amount 0 of probiotic consuming by nauplii was calculated. The significant enrichment was related to the treatment which fed with bakery yeast (541/95±44/79 CFU/nauplii) for 24 hours (P< 0.05). The significant survival rate (93%) was observed in treatment which enriched with Bacillus licheniformis for 24 hours (P< 0.05). The present study has shown that Artemia urmiana nauplii have a good potential of enrichment and the brine shrimp (Artemia urmiana) has different response to different probiotic and density.