Cellulose extracted from gebang (Corypha utan) leaves was characterized and used as a raw material for preparation of carboxymethyl cellulose which further used for preparing a film. Carboxymethyl Cellulose/polyvinyl alcohol films were prepared by the crosslinking method. The films were chemically crosslinked with citric acid for tuning their properties. The results indicate that the hydrogen bond interactions between carboxymethylcellulose/polyvinyl alcohol and citric acid can be formed. The films demonstrated excellent antibacterial effects against Escherichiacoli and Staphylococcusaureus. Increase the addition of citric acid can efficiently reduce the water absorption of the films. Moreover, the films present three-dimensional structure, porous networks and low toxicity. Therefore, the developed carboxymethyl cellulose/polyvinyl alcohol/citric acid film can be well-suited for biomedical application.