Extracts prepared from the isolated nuclei of cultured cells are functional and accurate in in
vitro transcription and mRNA processing. Thus, such extracts can be used directly for
functional studies and as the starting material for purification of the proteins involved in these
processes. In this unit, a procedure for generating reproducible extracts from different
preparations of nuclei is described. The conditions are optimized to produce transcriptionally
active extracts from HeLa cells. The protocol can also be optimized to increase the yield of …

Preparation of nuclear and cytoplasmic extracts from mammalian cells | CiNii Research …
Preparation of nuclear and cytoplasmic extracts from mammalian cells …