Primary capture of Bacillus subtilis xylanase from crude feedstock using alcohol/salt liquid biphasic flotation

PE Kee, L San Cheah, PK Wan, PL Show… - Biochemical …, 2021 - Elsevier
Liquid biphasic flotation (LBF) offers advanced downstream processing of bioproducts by
floating of gas bubbles within the biphasic system to enhance the separation efficiency of
target bioproducts. In this study, the feasibility of alcohol/salt LBF for primary capture of
extracellular Bacillus subtilis xylanase from crude feedstock was investigated. The effects of
types of phase-forming components; phase composition; concentration of crude feedstock
load; and flotation time on the recovery performance of xylanase in LBF were evaluated. The …
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