Process engineering studies of free and micro-encapsulated β-galactosidase in batch and packed bed bioreactors for production of galactooligosaccharides

P Sen, A Nath, C Bhattacharjee, R Chowdhury… - Biochemical …, 2014 - Elsevier
In the present investigation, an attempt has been made to produce galactooligosaccharides
(GOS) 1 from de-proteinated casein whey using free and immobilized β-galactosidase. An
extensive investigation on β-galactosidase immobilization by micro-encapsulation on
calcium alginate has been carried out to evaluate the optimum process parameters namely
sodium alginate to enzyme ratio (SER) 2 (100: 3) and calcium chloride concentration (0.3 M)
with an intention to get maximum enzyme loading and retention in activity. Investigations on …
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