Producing glucose 6-phosphate from cellulosic biomass: structural insights into levoglucosan bioconversion
The most abundant carbohydrate product of cellulosic biomass pyrolysis is the
anhydrosugar levoglucosan (1, 6-anhydro-β-d-glucopyranose), which can be converted to
glucose 6-phosphate by levoglucosan kinase (LGK). In addition to the canonical kinase
phosphotransfer reaction, the conversion requires cleavage of the 1, 6-anhydro ring to allow
ATP-dependent phosphorylation of the sugar O6 atom. Using x-ray crystallography, we
show that LGK binds two magnesium ions in the active site that are additionally coordinated …
anhydrosugar levoglucosan (1, 6-anhydro-β-d-glucopyranose), which can be converted to
glucose 6-phosphate by levoglucosan kinase (LGK). In addition to the canonical kinase
phosphotransfer reaction, the conversion requires cleavage of the 1, 6-anhydro ring to allow
ATP-dependent phosphorylation of the sugar O6 atom. Using x-ray crystallography, we
show that LGK binds two magnesium ions in the active site that are additionally coordinated …