Profiling APOL1 nephropathy risk variants in genome-edited kidney organoids with single-cell transcriptomics
Background DNA variants in APOL1 associate with kidney disease, but the pathophysiologic
mechanisms remain incompletely understood. Model organisms lack the APOL1 gene,
limiting the degree to which disease states can be recapitulated. Here we present single-cell
RNA sequencing (scRNA-seq) of genome-edited human kidney organoids as a platform for
profiling effects of APOL1 risk variants in diverse nephron cell types. Methods We performed
footprint-free CRISPR-Cas9 genome editing of human induced pluripotent stem cells …
mechanisms remain incompletely understood. Model organisms lack the APOL1 gene,
limiting the degree to which disease states can be recapitulated. Here we present single-cell
RNA sequencing (scRNA-seq) of genome-edited human kidney organoids as a platform for
profiling effects of APOL1 risk variants in diverse nephron cell types. Methods We performed
footprint-free CRISPR-Cas9 genome editing of human induced pluripotent stem cells …
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