Simple Summary The development of cost-effective strategies that can be easily
implemented on-farms is pivotal to promote a more judicious use of antimicrobials and its
reduction in livestock industry. Indeed, inappropriate use of antimicrobials is linked to the
phenomenon of antimicrobial resistance, a global health concern for both humans and
animals. Studies on other food-producing species have confirmed the effectiveness of
biosecurity measures on the reduction of antimicrobials, of while little is still known in beef …

Judicious antimicrobial stewardship in livestock industry is needed to reduce the use of
antimicrobials (AMU) and the associated risk of antimicrobial resistance. Biosecurity
measures are acknowledged for their role against the spread of diseases and the
importance in reducing AMU in different species. However, their effectiveness in beef
production has been scarcely considered. The aim of this study was to investigate the effect
of the quarantine strategy on AMU in beef cattle. A total of 1206 Charolaise animals in five …