The effects of copper and cadmium and the preparation Biomin (natural substance extracted from coal with ai humic acids) on the biometric parameters, some stress markers and enzymatic activities in leaves and roots of triticale plants were investigated. The functional carbon distribution in Biomin was analyzed by 13C NMR. It was found that all treatments retarded the growth and fresh weight of the plants. Heavy metals enhanced the content of stress markers proline and malondialdehyde and the activities of guaiacol peroxidase, superoxide dismutase and glutathione-S-transferase. The opposite trend was observed after Biomin application. Catalase activity was not affected considerably by the treatments. The free thiol-containing compounds were increased only in the roots of cadmium-treated plants. Comparative analysis of the measured parameters suggested that Biomin possessed protective effect against heavy metal toxicity.