Earthworms are now considered in animal feeds; especially as alternative for fish meal and source of protein. Based on these, therefore the need to study protozoan parasites becomes imperative due to zoonotic effect. One hundred and forty earthworms (75 of Alma millsoni and 65 of Libyodrilus violaceus) commonly found in Ago-Iwoye, Ogun State, Nigeria; were collected during the raining months of May, June, July and August 2006 by digging and hand sorting method. The worms were dissected and examined for protozoan and helminth parasites. Of the 75 individuals of A. millsoni sampled, 46 (61.3%) were infected with protozoans namely Apolocystis libyodrilii, Monocystis abegei, Monocystis libyodrilii, and L. violaceus had 83.1% prevalence of protozoan infection with an overall mean prevalence of of 72.2%. Chi-square analysis of the population of Apolocystis libyodrilii, Monocystis abegbei, and Monocystis libyodrili revealed a significant difference in Libyodrilus violaceus than in Alma millsoni at p<0.05. The present results of the study provide basic information on possible parasites of earthworms which may be transmitted to livestock or other animals when fed with these earthworm species. Potential vermiculturists need find ways of eliminating these parasites from the earthworms during processing of the worms for use as protein source.