Quantification of malondialdehyde in exhaled breath condensate using pseudo two-dimensional ultra-performance liquid chromatography coupled with single …
We developed a robust analytical method for quantification of malondialdehyde (MDA) in
exhaled breath condensate (EBC) via derivatization with 2, 4-dinitrophenylhydrazine
(DNPH). The target MDA-DNPH hydrazone was separated by ultra-performance liquid
chromatography using two reversed-phase analytical columns (C 18 and phenyl-hexyl) inter-
connected via a two-position, six-port switching valve to a single-quadrupole mass
spectrometer. The target derivative was analyzed under positive electrospray ionization …
exhaled breath condensate (EBC) via derivatization with 2, 4-dinitrophenylhydrazine
(DNPH). The target MDA-DNPH hydrazone was separated by ultra-performance liquid
chromatography using two reversed-phase analytical columns (C 18 and phenyl-hexyl) inter-
connected via a two-position, six-port switching valve to a single-quadrupole mass
spectrometer. The target derivative was analyzed under positive electrospray ionization …
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