Quantifying intracellular rates of glycolytic and oxidative ATP production and consumption using extracellular flux measurements

SA Mookerjee, AA Gerencser, DG Nicholls… - Journal of Biological …, 2017 - ASBMB
… Overall, we demonstrate how extracellular fluxes quantitatively reflect intracellular ATP
to calculate glycolytic and oxidative ATP production rates from raw extracellular acidification …

Quantifying intracellular rates of glycolytic and oxidative ATP production and consumption using extracellular flux measurements

SA Mookerjee, AA Gerencser, DG Nicholls… - Journal of Biological …, 2018 - ASBMB
… began to run glycolysis to lactate, leading to a 32% increase in the total ATP production rate
to 55.2 pmol of ATP/min/… This is equivalent to about 0.2 fmol of ATP/s/cell. At the same time, …

Determining maximum glycolytic capacity using extracellular flux measurements

SA Mookerjee, DG Nicholls, MD Brand - PloS one, 2016 - journals.plos.org
… Although full flux analysis using tracers can be used to quantify … total extracellular acidification
signal using oxygen consumption … The rates of ATP production by glycolysis and oxidative

Measurement and analysis of extracellular acid production to determine glycolytic rate

SA Mookerjee, MD Brand - Journal of visualized experiments …, 2015 - pmc.ncbi.nlm.nih.gov
… for measuring extracellular proton production rate (from … full oxidation of glucose,
mitochondrial Oxygen Consumption … , as well as their associated ATP production rates, can be …

[PDF][PDF] Calculating ATP production rates from oxidative phosphorylation and glycolysis during cell activation

BR Desousa, KKO Kim, WY Hsieh, AE Jones, P Swain… - bioRxiv, 2022 - scholar.archive.org
… We 4 therefore applied a method to quantify rates of ATP production from oxygen consumption
and 5 extracellular acidification data obtained from the Seahorse XF Analyzer to study …

[HTML][HTML] The contributions of respiration and glycolysis to extracellular acid production

SA Mookerjee, RLS Goncalves, AA Gerencser… - … et Biophysica Acta (BBA …, 2015 - Elsevier
… O 2 consumption and pH were monitored in a popular platform for measuring extracellular
… used to calculate the oxidative and glycolytic sources of ATP production as described above. …

Top‐down control analysis of ATP turnover, glycolysis and oxidative phosphorylation in rat hepatocytes

EK Ainscow, MD Brand - European Journal of Biochemistry, 1999 - Wiley Online Library
intracellular glucose-6-phosphate, pyruvate and ATP levels, … lactate dehydrogenase and
measuring oxidation of NADH (… of glycolysis, oxidative phosphorylation and ATP consumption

Calculation of ATP production rates using the Seahorse XF Analyzer

BR Desousa, KKO Kim, AE Jones, AB Ball… - EMBO …, 2023 - embopress.org
measuring real-time rates of ATP produced from oxidative phosphorylation and glycolysis
in … of the oxygen consumption rate (OCR; in pmol O 2 /min) and extracellular acidification …

Energy metabolism in astrocytes: high rate of oxidative metabolism and spatiotemporal dependence on glycolysis/glycogenolysis

L Hertz, L Peng, GA Dienel - Journal of Cerebral Blood Flow …, 2007 - journals.sagepub.com
… ), net synthesis of two more ATP occurs during glycolysis for … in vivo can be quantified by
use of specific labeled precursors … levels exceeding by 20- to 30-fold the content of intracellular

[PDF][PDF] Quantifying intracellular rates of glycolytic and oxidative ATP production and consumption using extracellular measurements

… Overall, we demonstrate how extracellular s quantitatively t intracellular ATP turnover and
glycolytic and oxidative ATP production rates from raw extracellular n and respiration data. …