SUMMARY A rapid, optimized, and sensitive reversed-phase high-performance liquid
chromatographic (RP-HPLC) method has been developed for determination of pentoxifylline
in human plasma. The analyte was extracted from the plasma with dichloromethane after
addition of 0.2 mL 1 M NaOH. HPLC separation was performed on a C18 analytical column
(250 mm× 4 mm id) with acetonitrile–water, 45: 55 (v/v), as mobile phase.
Spectrophotometric detection was performed at 275 nm. Calibration graphs were linear from …